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Table 1 Yield and percentage recovery of recombinant HIV-1 CA

From: Production and purification of polymerization-competent HIV-1 capsid protein p24 (CA) in NiCo21(DE3) Escherichia coli

Sample

Total protein (mg)

HIV CA (mg)

Purity (%)

Soluble lysate

288

39

13.5

Chitin beads

166

25

15.06

IMAC

17.5

17

97.1

  1. NiCo21(DE3)-pACYC-RIL expressing HIV-1 CA were grown in super broth the presence of 0.05 mM IPTG at 22°C for 12 hours and processed to purify recombinant protein as described in Methods. Quantities of recombinant HIV-1 CA are based on average values from three independent 100 mL expression cultures. HIV-1 CA was quantified by densitometry of bands on a western blots and the Qubit fluorometric total protein assay.
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BMC Biotechnology

ISSN: 1472-6750

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