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Figure 7 | BMC Biotechnology

Figure 7

From: Production and purification of polymerization-competent HIV-1 capsid protein p24 (CA) in NiCo21(DE3) Escherichia coli

Figure 7

FPLC and electrophoretic analysis of HIV-1 CA. HIV-1 CA was purified using TALON® Superflow™ cobalt-based IMAC column on an FPLC system. SDS/PAGE (12%) analysis results (inset) of the eluted fractions as shown in the FPLC chromatogram. Fractions A6 to A11 were pooled as the high purity product. Lane M, BenchMark Pre-stained protein ladder; Lanes W1/W2, unbound protein in wash fractions; Lanes A6-A11, CA eluted in imidazole gradient.

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BMC Biotechnology

ISSN: 1472-6750

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