Skip to main content
Figure 3 | BMC Biotechnology

Figure 3

From: C-terminal in Sp1-like artificial zinc-finger proteins plays crucial roles in determining their DNA binding affinity

Figure 3

Total protein and purified 7ZFP Active-H in different preparations from Rosetta DE3 bacteria transformed with the pET-28 7ZFP or empty control vectors. (a) Total crude proteins expressing 7ZFP (line-pointed band), Coomassie Blue staining. (b) The expression of 7ZFP Active-D was displayed in Western blot assay using antibody against 6 × Histidine-tag (Lane-1, total protein for pET-28a control vector; Lane-2, total protein for pET-28a-7ZFP vector; Lane-3, the sonicated supernatant for pET-28a-7ZFP vector; Lane-4, the sonicated precipitate for pET-28a-7ZFP vector; Lane-5, 7ZFP purified from the sonicated supernatant using Ni-NTA-Sepharose beads).

Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us