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Figure 5 | BMC Biotechnology

Figure 5

From: Engineering of the LukS-PV and LukF-PV subunits of Staphylococcus aureusPanton-Valentine leukocidin for Diagnostic and Therapeutic Applications

Figure 5

Ion Exchange Chromatography (IEC) of 6His-LukS-PV. A AKTA Explorer chromatogram during ion exchange purification of 6His-LukS-PV. NOTE: The blue curve is the entire chromatographic plot itself. On the vertical axis, mS/cm is the conductivity or absorbance, while the green signal is the pressure. On the horizontal axis, the numbers in red (upper) are the identification of the tubes containing the protein, while the grey numbers (lower) is the timer (minutes). B Purity of 6His-LukS-PV following purification by IEC. Lanes 1, 2, 3 & 4, All of the eluate had bands of impurity between the 33 kDa and 25 kDa Mw marks. Lane M, Protein Mw standard.

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