Table 4 Summary of specific biological activities for rSAK-2 from crude fermentation supernantant samples after harvest compared to the SAK reference standard from NIBSC
From: Expression of recombinant staphylokinase in the methylotrophic yeast Hansenula polymorpha
Scale/Sample | Measurement number | Concentration by PAA [μg/ml] | Activity by PAA [IU/ml] | Specific activity [IU/mg] | |
|---|---|---|---|---|---|
80 L scale | Batch 1 | 1 | 883.87 | 55.59 | 58.302 |
2 | 783.87 | ||||
Batch 2 | 1 | 649.03 | 42.32 | 58.376 | |
2 | 620.65 | ||||
Batch 3 | 1 | 816.13 | 52.42 | 56.232 | |
2 | 756.45 | ||||
2 L scale | 1 | 999.19 | 60.73 | 58.446 | |
2 | 822.58 | ||||
SAK STAR NIBSC 94/718 (1 IU = 15 μg as described in datasheet) | Theoretical value | 15 | 1 | 66.667 | |