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Table 4 Summary of specific biological activities for rSAK-2 from crude fermentation supernantant samples after harvest compared to the SAK reference standard from NIBSC

From: Expression of recombinant staphylokinase in the methylotrophic yeast Hansenula polymorpha

Scale/Sample Measurement number Concentration by PAA [μg/ml] Activity by PAA [IU/ml] Specific activity [IU/mg]
80 L scale Batch 1 1 883.87 55.59 58.302
2 783.87
Batch 2 1 649.03 42.32 58.376
2 620.65
Batch 3 1 816.13 52.42 56.232
2 756.45
2 L
scale
1 999.19 60.73 58.446
2 822.58
SAK STAR NIBSC 94/718 (1 IU = 15 μg as described in datasheet) Theoretical value 15 1 66.667