Figure 3
From: Expression of recombinant staphylokinase in the methylotrophic yeast Hansenula polymorpha
Western blot analysis of rSAK-2, expressed in SYN6 medium supplemented with 20 different plant peptones and a comparative fermentation without peptone. Results from a 1 ml micro scale batch fermentation in the BioLector® system (5 μL supernatant loaded). Final lanes loaded with THR-174 (~109 ng). The most prominent SAK-2 signals appeared for vegetable peptone (1) and wheat peptone (17).