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Table 1 Expression constructs and details of inserts

From: Use of anionic denaturing detergents to purify insoluble proteins after overexpression

Plasmid number Insert Protein Domain Flybase Protein ID and AA range Uniprot ID MW of fusion protein in kDa
165 chico PH chico-PA Q9XTN2 15.6
8-107
166 chico PTB chico-PA Q9XTN2 16.6
122-235
168 Pi3K92E catalytic Pi3K92E-PA P91634 45.7
726-1088  
171 PDK1 PH-like Pdk1-PA Q9W0V1 14.3
593-680  
172 Akt1 catalytic Akt1-PA Q8INB9 40.3
189-509  
173 rictor REM rictor-PA Q9VWJ6 12.1
827-896  
174 Tor kinase catalytic Tor-PA Q9VK45 36.3
2074-2352  
175 S6K catalytic S6k-PA P91656 39.2
92-402  
176 TSC2 Tuberin gig-PA Q9VW83 40.5
561-883  
177 TSC2 DUF 3384 gig-PA Q9VW83 54.3
37-473  
178 raptor WD raptor-PA Q9W437 47.9
1210-1624  
179 InR Y-kinase InR-PA P09208 36.7
1363-1625  
180 Akt1 PH-like Akt1-PA Q8INB9 16.0
26-129  
181 foxo winged helix foxo-PB Q95V55 13.1
95-175  
182 Tor kinase DUF 3385 Tor-PA Q9VK45 22.8
830-998  
183 Pi3K21B N-term SH2 Pi3K21B-PA O18683 16.6
24-133  
184 Pi3K92E accessory Pi3K92E-PA P91634 24.6
552-723
  1. All constructs are based on pET151/D-TOPO. This vector contains a 6xHis-V5 Epitope - TEV cleavage sequence tag of approximately 4 kDa. The molecular weights given are those of the entire fusion protein, i.e. containing the tag.