Use of anionic denaturing detergents to purify insoluble proteins after overexpression

BMC Biotechnology

Table 1 Expression constructs and details of inserts

Plasmid number	Insert Protein Domain	Flybase Protein ID and AA range	Uniprot ID	MW of fusion protein in kDa
165	chico PH	chico-PA	Q9XTN2	15.6
165	chico PH	8-107	Q9XTN2	15.6
166	chico PTB	chico-PA	Q9XTN2	16.6
166	chico PTB	122-235	Q9XTN2	16.6
168	Pi3K92E catalytic	Pi3K92E-PA	P91634	45.7
168	Pi3K92E catalytic	726-1088		45.7
171	PDK1 PH-like	Pdk1-PA	Q9W0V1	14.3
171	PDK1 PH-like	593-680		14.3
172	Akt1 catalytic	Akt1-PA	Q8INB9	40.3
172	Akt1 catalytic	189-509		40.3
173	rictor REM	rictor-PA	Q9VWJ6	12.1
173	rictor REM	827-896		12.1
174	Tor kinase catalytic	Tor-PA	Q9VK45	36.3
174	Tor kinase catalytic	2074-2352		36.3
175	S6K catalytic	S6k-PA	P91656	39.2
175	S6K catalytic	92-402		39.2
176	TSC2 Tuberin	gig-PA	Q9VW83	40.5
176	TSC2 Tuberin	561-883		40.5
177	TSC2 DUF 3384	gig-PA	Q9VW83	54.3
177	TSC2 DUF 3384	37-473		54.3
178	raptor WD	raptor-PA	Q9W437	47.9
178	raptor WD	1210-1624		47.9
179	InR Y-kinase	InR-PA	P09208	36.7
179	InR Y-kinase	1363-1625		36.7
180	Akt1 PH-like	Akt1-PA	Q8INB9	16.0
180	Akt1 PH-like	26-129		16.0
181	foxo winged helix	foxo-PB	Q95V55	13.1
181	foxo winged helix	95-175		13.1
182	Tor kinase DUF 3385	Tor-PA	Q9VK45	22.8
182	Tor kinase DUF 3385	830-998		22.8
183	Pi3K21B N-term SH2	Pi3K21B-PA	O18683	16.6
183	Pi3K21B N-term SH2	24-133		16.6
184	Pi3K92E accessory	Pi3K92E-PA	P91634	24.6
184	Pi3K92E accessory	552-723	P91634	24.6

All constructs are based on pET151/D-TOPO. This vector contains a 6xHis-V5 Epitope - TEV cleavage sequence tag of approximately 4 kDa. The molecular weights given are those of the entire fusion protein, i.e. containing the tag.

ISSN: 1472-6750