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Table 3 PCR analysis of MC1 plants

From: The Bxb1 recombination system demonstrates heritable transmission of site-specific excision in Arabidopsis

MC1-Parent line Plants tested Positive for target locus a Positive for recombinase gene b Positive for excision and recombinase genec Positive for
excision and negative for unexcised product d
Bxb1.9.17 31 30 29 28 28
Bxb1.9.31 31 31 19 19 19
Bxb1.12.69 16 16 14 14 14
Bxb1.12.72 16 15 7 7 3
Bxb1.35.15 28 26 25 24 24
Bxb1.35.33 46 45 36 35 13
  1. a Primers eand fyielded the 1.23 and/or 0.43 kb target fragment
  2. b Primers gand hyielded the 0.72 kb Bxb1 fragment
  3. c Primers eand fyielded the 0.43 kb excision fragment and primers gand hdetect the 0.72 kb Bxb1 fragment
  4. d Primers eand fyielded the 0.43 kb excision fragment and failed to detect a 1.23 kb target fragment