The Bxb1 recombination system demonstrates heritable transmission of site-specific excision in Arabidopsis

BMC Biotechnology

Table 3 PCR analysis of MC₁ plants

MC₁-Parent line	Plants tested	Positive for target locus ^a	Positive for recombinase gene ^b	Positive for excision and recombinase gene^c	Positive for excision and negative for unexcised product ^d
Bxb1.9.17	31	30	29	28	28
Bxb1.9.31	31	31	19	19	19
Bxb1.12.69	16	16	14	14	14
Bxb1.12.72	16	15	7	7	3
Bxb1.35.15	28	26	25	24	24
Bxb1.35.33	46	45	36	35	13

^a Primers eand fyielded the 1.23 and/or 0.43 kb target fragment
^b Primers gand hyielded the 0.72 kb Bxb1 fragment
^c Primers eand fyielded the 0.43 kb excision fragment and primers gand hdetect the 0.72 kb Bxb1 fragment
^d Primers eand fyielded the 0.43 kb excision fragment and failed to detect a 1.23 kb target fragment

ISSN: 1472-6750