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Table 1 PCR analysis of TR1 plants

From: The Bxb1 recombination system demonstrates heritable transmission of site-specific excision in Arabidopsis

TA Parent line Plants tested Positive for
recombinase gene aand target locus b
Positive for excision c Positive for
excision and negative for unexcised productd
Bxb1.9 48 28 28 17
Bxb1.12 78 31 28 26
Bxb1.28 18 18 18 16
Bxb1.35 44 28 26 13
  1. a Primers gand hyielded the 0.72 kb Bxb1 fragment
  2. b Primers eand fyielded the 1.23 and/or 0.43 kb fragment
  3. c Primers eand fyielded the 0.43 kb excision fragment
  4. d Primers eand ffailed to detect a 1.23 kb target fragment