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Table 1 PCR analysis of TR1 plants

From: The Bxb1 recombination system demonstrates heritable transmission of site-specific excision in Arabidopsis

TA Parent line

Plants tested

Positive for

recombinase gene aand target locus b

Positive for excision c

Positive for

excision and negative for unexcised productd

Bxb1.9

48

28

28

17

Bxb1.12

78

31

28

26

Bxb1.28

18

18

18

16

Bxb1.35

44

28

26

13

  1. a Primers gand hyielded the 0.72 kb Bxb1 fragment
  2. b Primers eand fyielded the 1.23 and/or 0.43 kb fragment
  3. c Primers eand fyielded the 0.43 kb excision fragment
  4. d Primers eand ffailed to detect a 1.23 kb target fragment
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BMC Biotechnology

ISSN: 1472-6750

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