Figure 4

S ₁ plants examined by Southern blot analysis and whole plant genomic PCR. (a) Genomic DNA cleaved with EcoRV hybridized with a ³²P-labeled GUS1350 probe (Figure 1). (b) Genomic DNA PCR amplified with with primers e and f. Plant lines (lanes 1, 2) S₁-Bxb1.9; (lanes 3, 4) S₁-Bxb1.12; (lane 5, 6) S₁-Bxb1.28; (lane 7, 8) S₁-Bxb1.35. Control lanes are wt (wild type Arabidopsis DNA) and TA₃-Bxb1.9, (target lines).