Table 3 GUS activity observed in cotton callus transformed with constructs carrying synJ and its modified versions MsynJ and synM, downstream to 35S promoter
From: A 28 nt long synthetic 5′UTR (synJ) as an enhancer of transgene expression in dicotyledonous plants
Construct code | No. of calli analyzed | GUS activity (pmolMU/min/μg protein) | ||||
|---|---|---|---|---|---|---|
Range | Percentage of callus showing activity in the range of | |||||
< 500 | 500-1000 | 1000-2000 | > 2000 | |||
35S(synJ) | 20 | 98.9-6490.4 | 20.0 | 15.0 | 20.0 | 45.0 |
35S(MsynJ) | 22 | 3.2-1278.1 | 50.0 | 36.4 | 13.6 | 0 |
35S(synM) | 14 | 5.2-802.4 | 78.6 | 21.4 | 0 | 0 |