Potential of primary kidney cells for somatic cell nuclear transfer mediated transgenesis in pig

BMC Biotechnology

Table 3 Nanofection results PKC2109

DNA amount [μg]	Nanofectin [μl]	Quality	Cells in suspension	Fluor. intensity	Transfection efficiency [%]
0.5	1.2	1	0	+	10
	2.0	1	0	+	16
	3.2	1	1	+	16
	4.0	1	1	+	15
1.0	1.2	2	1	+	25
	2.0	2	1	+	17
	3.2	2	1	++	21
	4.0	3	1	+	20
1.5	2.0	3	1	+	7
1.5	3.2	3	1	++	11

Scale for the assessment of cell quality: 1- excellent to good; 2- no vacuoles and lipids detectable, cells are bit stretched; 3- partially vacuoles and lipids, stretched cells; Scale for the assessment of cells in suspension: 0- none; 1- low number of cells; 2- a lot of cells; Fluor. = Fluorescence.

ISSN: 1472-6750

DNA amount [μg]	Nanofectin [μl]	Quality	Cells in suspension	Fluor. intensity	Transfection efficiency [%]
0.5	1.2	1	0	+	10
	2.0	1	0	+	16
	3.2	1	1	+	16
	4.0	1	1	+	15
1.0	1.2	2	1	+	25
	2.0	2	1	+	17
	3.2	2	1	++	21
	4.0	3	1	+	20
1.5	2.0	3	1	+	7
1.5	3.2	3	1	++	11

DNA amount [μg]	Nanofectin [μl]	Quality	Cells in suspension	Fluor. intensity	Transfection efficiency [%]
0.5	1.2	1	0	+	10
	2.0	1	0	+	16
	3.2	1	1	+	16
	4.0	1	1	+	15
1.0	1.2	2	1	+	25
	2.0	2	1	+	17
	3.2	2	1	++	21
	4.0	3	1	+	20
1.5	2.0	3	1	+	7
1.5	3.2	3	1	++	11

DNA amount [μg]	Nanofectin [μl]	Quality	Cells in suspension	Fluor. intensity	Transfection efficiency [%]
0.5	1.2	1	0	+	10
	2.0	1	0	+	16
	3.2	1	1	+	16
	4.0	1	1	+	15
1.0	1.2	2	1	+	25
	2.0	2	1	+	17
	3.2	2	1	++	21
	4.0	3	1	+	20
1.5	2.0	3	1	+	7
1.5	3.2	3	1	++	11