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Table 3 Nanofection results PKC2109

From: Potential of primary kidney cells for somatic cell nuclear transfer mediated transgenesis in pig

DNA amount [μg] Nanofectin [μl] Quality Cells in suspension Fluor. intensity Transfection efficiency [%]
0.5 1.2 1 0 + 10
2.0 1 0 + 16
3.2 1 1 + 16
4.0 1 1 + 15
1.0 1.2 2 1 + 25
2.0 2 1 + 17
3.2 2 1 ++ 21
4.0 3 1 + 20
1.5 2.0 3 1 + 7
3.2 3 1 ++ 11
  1. Scale for the assessment of cell quality: 1- excellent to good; 2- no vacuoles and lipids detectable, cells are bit stretched; 3- partially vacuoles and lipids, stretched cells; Scale for the assessment of cells in suspension: 0- none; 1- low number of cells; 2- a lot of cells; Fluor. = Fluorescence.