Figure 2
From: A designer hyper interleukin 11 (H11) is a biologically active cytokine
Purification of H11-SDS-PAGE (A) and Western Blot (B) analysis. The proteins were visualized with Roti-Blue stain and for detection H11 the anti- IL-11Rα antibody (Santa Cruz Biotechnology, Santa Cruz, CA) was used. Lane 1- molecular weight marker PageRuler (Fermentas), lane 2-supernatant from infected High-Five BTI-TN-5B1-4 cells, 3-supernatant diluted with 20 mM 1.3 DAP (1:8), 4-eluted H11.