Figure 1
From: Live attenuated influenza viruses produced in a suspension process with avian AGE1.CR.pIX cells
TCID 50 titers (open symbols) and HA yields (closed symbols) from infections performed with different medium exchange strategies. CR.pIX cells were infected with influenza strain A/PR/8/34 in shaker flasks at a CCI of 2 × 106 cells/mL and 1 × 10-6 units trypsin per cell. Three strategies were performed: infection without medium exchange (■, black), after a medium exchange (▲, cyan) and after the exchange of 50% of the medium (●, purple). Error bars indicate standard deviation of three independent cultures.