Skip to main content

Table 1 Analysis of EuIPI enzymatic activity

From: Overexpression of an isopentenyl diphosphate isomerase gene to enhance trans-polyisoprene production in Eucommia ulmoides Oliver

 

Background (no enzyme)

EuIPI (5 μg)

[4-14C] IPP Incorporation (DPM)

65.92 ± 2.86

417.19 ± 4.86

  1. For EuIPI enzymatic activity assay, the 50-μL reaction mixture contained 10 nmol [4-14C] IPP (37 GBq/mol) substrate and 5 μg purified protein (0.5 μg/μL). Radioactivity of reacted products was measured with a liquid scintillation counter as described in the Materials and methods. Distilled water (no enzyme) was used for background measurement; Data represent means ± standard error, n=3.
Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us