From: Cloning, sequence analysis, expression of Cyathus bulleri laccase in Pichia pastoris and characterization of recombinant laccase
Purification Steps
Total activity (U)
Total protein (mg)
Specific activity (U/mg)
Yield (%)
Purification fold
Crude extract
370
18
20.5
100
1
Ammonium sulfate precipitation
138.24
3.6
38.4
37.3
~1.9
Sephadex G75 + SuperdexG200
90
1.06
84.9
24.3
~4.0