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Table 1 Summary of purification of laccase secreted by P. pastoris pPICZ╬▒B lcc -5

From: Cloning, sequence analysis, expression of Cyathus bulleri laccase in Pichia pastoris and characterization of recombinant laccase

Purification Steps Total activity (U) Total protein (mg) Specific activity (U/mg) Yield (%) Purification fold
Crude extract 370 18 20.5 100 1
Ammonium sulfate precipitation 138.24 3.6 38.4 37.3 ~1.9
Sephadex G75 + SuperdexG200 90 1.06 84.9 24.3 ~4.0