Figure 4

Reductive metabolism of Vero-B4 cells after cold storage and rewarming. Vero-B4 cells were incubated at 4°C for 168 hours in RPMI 1640, Krebs-Henseleit (KH) buffer containing glucose (KHG), or modified buffers containing a low Ca²⁺ concentration (KHG(Ca-)), a high inorganic phosphate concentration (KHG(P+)) or different combinations thereof (KHG(Ca-,P+), (KH(Ca-,P+)) and then rewarmed in RPMI 1640 at 37°C for 3 hours. Some cells were incubated at 4°C in the presence of the iron chelator deferoxamine (1 mM; striped bars). The resazurin reduction assay was performed after the rewarming period (171 h of incubation). Resazurin reduction was expressed as percentage of that of non-stored control cells (n = 4; * p < 0.01 vs. KHG).