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Figure 2 | BMC Biotechnology

Figure 2

From: A one-step cloning method for the construction of somatic cell gene targeting vectors: application to production of human knockout cell lines

Figure 2

Construction of a somatic cell KO vector via a one-step cloning method . Cassette A is indicated by two rectangles and cassette B by two echelons. The homologous arms were amplified using PCR with primers containing the 15–18 nt sequence at the 5′ end and sequences specific for the ExoIII-generated overhangs in pAAV-LIC. E2–6: exons 2–6; NEO: neomycin resistance gene; TK: thymidine kinase promoter.

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