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Table 3 Values of independent variables at different levels of CCD design

From: Enhanced cell disruption strategy in the release of recombinant hepatitis B surface antigen from Pichia pastoris using response surface methodology

Independent variable Levels
  -α (1.682) −1 0 +1 +α (1.682)
Number of passes (times) 12 15 20 25 28
Biomass concentration (g/L) 4.41 5.74 7.70 9.65 10.98
Pulse pressure (bar) 327.28 600 1,000 1,400 1672.72