From: Structural insights from random mutagenesis of Campylobacter jejunioligosaccharyltransferase PglB
PglB variant | ELISA EPA-CP5 | PglB polypeptide | |
---|---|---|---|
 | Abs. 450 nm | % of wt | anti-HA, % of wt |
wt | 0.57 (±0.03) | 100 | 100 (±13) |
mut | 0.20 (±0.01) | 0 | 56 (±7) |
I571V | 0.51 (±0.05) | 85 | 89 (±28) |
I571V V575I | 0.52 (±0.04) | 87 | 85 (±11) |
I571V V575L | 0.46 (±0.04)* | 70 | 84 (±7) |
I571F V575L | 0.32 (±0.01)* | 34 | 96 (±31) |
I571C V575I | 0.41 (±0.03)* | 58 | 65 (±13) |