Figure 2
From: Structural insights from random mutagenesis of Campylobacter jejunioligosaccharyltransferase PglB
Screening of OST mutant libraries. Sorted ELISA read-outs of 96-well random mutant libraries of PglB Cj. Open circles: epPCR mutagenesis of the periplasmic domain (H423-I713), inactive clones selected for anti-HA screening and sequencing marked by an elipsoid. Open triangles: Cassette mutagenesis of I571 and V575 using primers with NDT degeneracy, active clones selected for sequencing marked by an elipsoid. Absorbance at 450 nm was measured against air. Filled symbols: wild-type controls on same plate (pGVXN112Kan), shaded symbols: inactive (mut) controls on same plate (pGVXN113Kan), host strain: E. coli CLM24 (pGVXN150-pGVXN345).