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Figure 3 | BMC Biotechnology

Figure 3

From: In-house preparation of hydrogels for batch affinity purification of glutathione S-transferase tagged recombinant proteins

Figure 3

Elution and Binding of PEGDA:GSH compared to PEGDA:Cys. A: Gross fluorescence observation of the gels confirmed GST-GFP association with homogenates prepared with PEGDA:GSH ratio (top) or equimolar PEGDA:Cys (bottom). B: Gross fluorescence observation of the gels confirmed PEGDA:GSH homogenates eluted with either 10 mM cysteine (bottom) or 10 mM GSH (top). C: SDS PAGE electrophoresis confirmed that minimal protein eluted from (lanes 1–3) PEGDA gels eluted with GSH, (lanes 4–6) PEGDA:Cys gels eluted with GSH, but protein did elute (lanes 7–9) from PEGDA:GSH gels eluted with GSH. The last lane is a protein ladder, arrows indicating 100 kDa, and 55 kDa.

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BMC Biotechnology

ISSN: 1472-6750

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