Figure 2

Affinity of GST-GFP to PEGDA:GSH homogenates. A: Influence of varying PEGDA:GSH ratio on binding of protein as expressed by the amount (μg) of protein in solution following 2 h incubation in the presence of PEGDA-GSH hydrogel homogenate (blue bars) or matched GSH-free PEGDA hydrogel (red bars). B: Influence of PEGDA:GSH ratio on specifically bound, or incorporated, GST-GFP as expressed as percent total GST. Total protein (100%) was 60 μg GST-GFP for all groups. † for p < 0.05, †† for p < 0.01, and ††† for p < 0.001. Bars and points represent the mean of 3 independents samples plus or minus (±) standard deviation.