Figure 9

Masson’s trichrome stain. A: 3 wk healing after both right and left sides received B-247 treatment of TCP loaded then lyophilized with 50 μg/ml rhBMP-2 plus 100 μg/ml of pln.247 plasmid. Inset arrow designates region of panel B. B: 400x magnification of region designated in panel A between existing maxilla (bottom) and new osteoid with marrow space and partially integrated TCP. A line of demarcation can be discerned between the labels Osteoid and Maxilla. C: 6 wk healing; Left - B-247 treatment of TCP loaded and lyophilized with 50 μg/ml rhBMP-2 plus 100 μg/ml of pln.247 plasmid. Right - TCP loaded and lyophilized with 50 μg/ml BMP-2. No new bone was generated by the TCP particles activated with only the low-dose BMP-2 (left). Hashed regions represent the original maxillary ridge. C: 6 wk healing; Left - TCP loaded and lyophilized with 50 μg/ml rhBMP-2 plus 100 μg/ml of the empty-vector plasmid pBI. Right - TCP loaded and lyophilized with 50 μg/ml BMP-2. Area encircled represents new bone (blue) generated by the bioactivated TCP particles. Bar = 0.5 mm. Pairs of images for B and C are from same animal. Bar = 0.5 mm.