Skip to main content

Table 3 Effect of detergents and enzyme inhibitors on esterase activity

From: Cloning and characterization of a novel 2-ketoisovalerate reductase from the beauvericin producer Fusarium proliferatum LF061

Compounds Concentration Relative activity (%)
Control 0 100.0 ± 2.1
DTT 3 mM 126.9 ± 4.9
CTAB 3 mM 42.7 ± 2.2
DEPC 3 mM 113.6 ± 2.7
PMSF 3 mM 53.6 ± 4.1
SDS 3 mM 6.3 ± 1.9
β-mercaptoethanol 0.5% 101.7 ± 2.6
Triton X-100 0.5% 60.8 ± 4.6
Tween-80 0.5% 176.4 ± 2.1
Tween-20 0.5% 128.7 ± 3.3
  1. Activity without detergents and enzyme inhibitors was set as 100%. All measurements were repeated three times.