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Table 3 Effect of detergents and enzyme inhibitors on esterase activity

From: Cloning and characterization of a novel 2-ketoisovalerate reductase from the beauvericin producer Fusarium proliferatum LF061

Compounds

Concentration

Relative activity (%)

Control

0

100.0 ± 2.1

DTT

3 mM

126.9 ± 4.9

CTAB

3 mM

42.7 ± 2.2

DEPC

3 mM

113.6 ± 2.7

PMSF

3 mM

53.6 ± 4.1

SDS

3 mM

6.3 ± 1.9

β-mercaptoethanol

0.5%

101.7 ± 2.6

Triton X-100

0.5%

60.8 ± 4.6

Tween-80

0.5%

176.4 ± 2.1

Tween-20

0.5%

128.7 ± 3.3

  1. Activity without detergents and enzyme inhibitors was set as 100%. All measurements were repeated three times.
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BMC Biotechnology

ISSN: 1472-6750

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