Figure 7

Effect of pH on the purified ketoisovalerate reductase KivRFp. Relative activity of ketoisovalerate reduction was performed in various pH buffers at 35°C (pH 5.0-8.0, 50 mM sodium phosphate buffer; pH 8.0-10.5, 50 mM Tris–HCl buffer). The activity at pH 7.5 was set as 100%. All measurements were performed in triplicate.