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Table 1 Freezing protocols used

From: Effect of different freezing rates during cryopreservation of rat mesenchymal stem cells using combinations of hydroxyethyl starch and dimethylsulfoxide

Protocol Description Duration [min] References
1 0,3°C/min to −100°C 347  
  Store in vapour phase at −134°C   [47]*
2 1°C/min to −80°C 84 [4650]
  Store in vapour phase at −134°C   
3 1°C/min to - 30°C 44  
  5°C/min to −80°C   [44, 51]*
  xStore in vapour phase at −134°C   
4 1°C/min to −20°C 34  
  5°C/min to −40°C   
  10°C/min to −80°C   Designed
  20°C/min to −100°C   
  Store in vapour phase at −134°C   
5 1°C/min to −6°C 26  
  25°C/min to −50°C   Designed
  10°C/min to −90°C   
  Store in vapour phase at −134°C   
6 Directly into the vapour phase 0  
7 99°C/min to −100°C 2  
  Store in vapour phase at −134°C   [35, 52]*
  1. * Modified.