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Table 1 Analysis of transfection efficiency of human primary calvarial suture mesenchymal cells by lipid-based transfection methods

From: Development of an efficient, non-viral transfection method for studying gene function and bone growth in human primary cranial suture mesenchymal cells reveals that the cells respond to BMP2 and BMP3

Transfection Method

% Cell Survival

% Transfection Efficiency

Turbofect

ND

1.5±0.2

Lipofectamine 2000

ND

1.6±0.1

DOTAP

ND

1.4±0.1

X-Fect

ND

4.1±0.1

Endofectine

64.9±8.5

2.8±0.6

Metafectine

89.6±12.1

3.5±0.3

  1. ND = not determined. Values ± SD represent the mean of 3 biological replicates.
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BMC Biotechnology

ISSN: 1472-6750

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