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Table 2 The effects of the ‘line’, ‘transgene’ and ‘ontogeny’ factors on the fruit quality in the sweet orange lines

From: Field performance of transgenic citrus trees: Assessment of the long-term expression of uidA and nptIItransgenes and its impact on relevant agronomic and phenotypic characteristics

Source Fruit quality parameter (dependent variable)
  Weight Volume Caliber Color Index Juice content1 TA TSS MI
  S1 S2 S3 S1 S2 S3 S1 S2 S3 S1 S2 S3 S1 S2 S3 S1 S2 S3 S1 S2 S3 S1 S2 S3
Factor / ANOVA 2                         
Line NS NS NS NS NS NS NS * NS NS * NS * NS NS *** *** *** NS NS NS * * ***
Plant (Line) *** *** *** *** *** *** *** *** *** *** *** *** * * NS *** *** *** *** *** *** *** *** NS
Contrasts to test ‘transgene’ effect 3                         
P3A vs PCA NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS *
P4A vs PCA NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS **
P5A vs PCA NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS
P6A vs PCA NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS
P7A vs PCA NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS
P8A vs PCA NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS NS
Contrasts to test ‘ontogeny’ effect 4                         
J vs A NS * NS NS ** NS NS ** NS NS ** NS NS NS NS *** *** *** NS NS NS ** ** ***
PCJ vs PCA NS * NS NS * NS NS * NS NS ** NS NS NS NS *** * *** NS NS NS ** NS ***
TJ vs TA NS NS NS NS NS NS NS * NS NS * NS NS NS NS * *** *** NS NS NS NS ** ***
  1. 1The “juice content” variable was log-transformed prior to the analyses to fit the data to a normal distribution.
  2. 2ANOVA to test the effects of Line on each fruit quality variable. Independent statistical analyses were performed for each fruit quality parameter and season.
  3. 3Contrasts to test for significant differences between each adult transgenic line and their respective control line (PCA) using Dunnett’s test.
  4. 4Planned comparisons to test for significant differences between juvenile and adult lines. J, average of all juvenile lines; A, average of all adult lines; TJ, average of all juvenile and transgenic lines; TA, average of all adult and transgenic lines.
  5. TA, titratable acidity; TSS, total soluble solids; MI, maturity index (TSS/TA); S1, season 2004; S2, season 2005; S3, season 2006.
  6. *p < 0.01; **p < 0.001; ***p < 0.0001; NS, not significant.
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