Source
|
Fruit quality parameter (dependent variable)
|
---|
|
Weight
|
Volume
|
Caliber
|
Color Index
|
Juice content1
|
TA
|
TSS
|
MI
|
---|
|
S1
|
S2
|
S3
|
S1
|
S2
|
S3
|
S1
|
S2
|
S3
|
S1
|
S2
|
S3
|
S1
|
S2
|
S3
|
S1
|
S2
|
S3
|
S1
|
S2
|
S3
|
S1
|
S2
|
S3
|
---|
Factor / ANOVA
2
| | | | | | | | | | | | | | | | | | | | | | | | |
Line
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
*
|
NS
|
NS
|
*
|
NS
|
*
|
NS
|
NS
|
***
|
***
|
***
|
NS
|
NS
|
NS
|
*
|
*
|
***
|
Plant (Line)
|
***
|
***
|
***
|
***
|
***
|
***
|
***
|
***
|
***
|
***
|
***
|
***
|
*
|
*
|
NS
|
***
|
***
|
***
|
***
|
***
|
***
|
***
|
***
|
NS
|
Contrasts to test ‘transgene’ effect
3
| | | | | | | | | | | | | | | | | | | | | | | | |
P3A vs PCA
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
*
|
P4A vs PCA
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
**
|
P5A vs PCA
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
P6A vs PCA
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
P7A vs PCA
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
P8A vs PCA
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
Contrasts to test ‘ontogeny’ effect
4
| | | | | | | | | | | | | | | | | | | | | | | | |
J vs A
|
NS
|
*
|
NS
|
NS
|
**
|
NS
|
NS
|
**
|
NS
|
NS
|
**
|
NS
|
NS
|
NS
|
NS
|
***
|
***
|
***
|
NS
|
NS
|
NS
|
**
|
**
|
***
|
PCJ vs PCA
|
NS
|
*
|
NS
|
NS
|
*
|
NS
|
NS
|
*
|
NS
|
NS
|
**
|
NS
|
NS
|
NS
|
NS
|
***
|
*
|
***
|
NS
|
NS
|
NS
|
**
|
NS
|
***
|
TJ vs TA
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
NS
|
*
|
NS
|
NS
|
*
|
NS
|
NS
|
NS
|
NS
|
*
|
***
|
***
|
NS
|
NS
|
NS
|
NS
|
**
|
***
|
- 1The “juice content” variable was log-transformed prior to the analyses to fit the data to a normal distribution.
- 2ANOVA to test the effects of Line on each fruit quality variable. Independent statistical analyses were performed for each fruit quality parameter and season.
- 3Contrasts to test for significant differences between each adult transgenic line and their respective control line (PCA) using Dunnett’s test.
- 4Planned comparisons to test for significant differences between juvenile and adult lines. J, average of all juvenile lines; A, average of all adult lines; TJ, average of all juvenile and transgenic lines; TA, average of all adult and transgenic lines.
- TA, titratable acidity; TSS, total soluble solids; MI, maturity index (TSS/TA); S1, season 2004; S2, season 2005; S3, season 2006.
- *p < 0.01; **p < 0.001; ***p < 0.0001; NS, not significant.