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Figure 5 | BMC Biotechnology

Figure 5

From: Development of an optimized tetracycline-inducible expression system to increase the accumulation of interleukin-10 in tobacco BY-2 suspension cells

Figure 5

Comparison of DsRed and vIL-10 accumulation levels. (A) Cell suspension cultures from different lines were induced with 10 μM Ahtc and 2-ml aliquots were analyzed daily from 3 to 7 dpi. The accumulation of vIL-10 in cell pellet extracts was determined by ELISA. (B) DsRed accumulation was determined in callus and cell suspension culture extracts from each transgenic line arrayed in 96-well plates using a fluorometer. The data reported are means ± SD from three independent experiments. (C) Linear regression between the yields of IL-10 (in suspension cultures at 7 dpi) and DsRed (in callus) for nine clones. The correlation coefficient R2 is indicated inside the plot.

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BMC Biotechnology

ISSN: 1472-6750

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