Figure 1From: Development of an optimized tetracycline-inducible expression system to increase the accumulation of interleukin-10 in tobacco BY-2 suspension cellsNorthern blot analysis of independent BY-2 callus clones transformed with pBin-TetR. Approximately 10 μg of total RNA was loaded (except for #6 and #25 due to limited amounts of biomass) and hybridized with a 32P-labeled TetR probe. Numbers indicate the different clones. Top panels: images of the hybridized blot as visualized on a phosphorimager. Bottom panels: agarose gels stained with ethidium bromide to show intact 28 S and 18 S rRNA bands.Back to article page