Concept of ABI-REC cloning. (A) A brief description of the principle of ABI-REC. For details, please refer to text (Results). The green rectangle represents the fusion bridge. The red rectangle represents the upstream homolog arm. The grey rectangle represents the insert of interest. The yellow triangle represents the pre-selected fusion site. The lowercase m represents methylated nucleotides within the plasmid. (B) Time schematic. ABI-REC allows the rapid creation of recombinant plasmids within one day.