Table 1 List of oligonucleotides used in this work
No | Name | Sequence |
|---|---|---|
1 | PZPN-1/Kpn2Ifor | GGCTCCGGAGAATGAACGCCAA |
2 | PZPN-1rev | TTCGATCAGCGGTTGCTTGCCA |
3 | PZPN-2for | TCGTGGCAAGCAACCGCTGAT |
4 | PZPN-2/XhoIrev | GCCTCGAGAGGCCGACGCGA |
5 | PZPN-1/Kpn2Ifor | GGCTCCGGAGAATGAACGCCAA |
6 | PZPN-2/XhoIrev | GCCTCGAGAGGCCGACGCGA |
7 | PZP2/XhoIfor | CAGACTCGAGTGTACTGAGAGTGCAC |
8 | PZP2/Kpn2Irev | AACGTCCGGAGCCGACTGCACTATAGCA |
9 | P500Kp1Nseqfor | ATTCGAGCTCGGTACCCGGG |
10 | P500N2seqfor | TTAGCGGCTAAAGGAGGCGG |
11 | P500Xh3Nseqfor | AGGTCTCTTTCCTGTGGATAGC |
12 | P500for | GCCCGAGGCATAGACTGTAC |
13 | P500rev | GCATCAGACAAACCGGCCAG |
14 | PZP500Mphfor | GAATGCATCACAGGCAGCAACGCT |
15 | PZP500Mphrev | GCGTGCATAATAAGCCCTACA |
16 | KanforMph | CAGCATCATGCATAATTGTGGTTTCA |
17 | KanrevMph | GTTGCGATGCATCTAGGTACTAAAACAAT |
18 | pPDF2.1EcoFor | AATGAATTCCAGAATGAGTTGTCA |
19 | pPDF2.1NcoRev | CATAGAGAACTCCATGGTTGGAGAAAG |
20 | RedforBspHI | AGATCATGACCTCCTCCGAGAAC |
21 | RedrevBam | AGTCGGATCCGCTAGAGGAACAGGT |
22 | DsRedfor2 | CCACCACCTGTTCCTCTAGC |
23 | Linker1 | AGCTGAATTCACGTGGTACCGCGGCCGCAGATCTA |
24 | Linker2 | AGCTTAGATCTGCGGCCGCGGTACCACGTGAATTC |
25 | P500terfor | GCTGGTGGCAGGATATATTG |
26 | P500RBseqrev | TTAAACTGAAGGCGGGAAAC |
27 | DsRedMfor | CAGAAGAAGGACAATGGGCTGGGA |
28 | DsREDnosTERfor | ACCTGTTCCTCTAGGATCGTTTCAAAC |
29 | DsREDnosTERrev | TTTGAACGATCCTAGAGGAACAGGTG |
30 | nosTERrevEco | TGAGAATTCAGAGATCTAGTAACATAG |