Figure 5

Proliferation potential of hADSCs within RH and CGH matrices. A) Phase contrast micrographs of MTT-stained viable hADSCs embedded in either RH or CGH matrices: UP: Phase contrast micrographs of MTT-stained viable hADSCs embedded in RH and CGH matrices after 2 days of culture showing a higher number of living cells in CGH matrix compared to RH matrix. DOWN: Phase contrast micrographs of MTT-stained viable hADSCs embedded in RH and CGH matrices after 7 days of culture showing an increasing number of living cells in both constructs as a result of cellular proliferation. B) hADSCs proliferation within RH and CGH matrices, measured by formazan absorbance: 2 and 7-days post-seeding. The proliferation rate in CGH matrix at 2 days of culture is 43.3% higher as compared to the RH matrix. At 7 days post-seeding CGH matrix contains 44.6% more viable cells than RH matrix. Both culture systems ensured a proliferation of approximately 17% at 7 days post-seeding as compared to a culture of 2 days. Results are presented as means ± SD (n = 3).