Development of efficient catharanthus roseus regeneration and transformation system using agrobacterium tumefaciens and hypocotyls as explants

Table 3 Experimental design for kanamycin concentration optimization

Treatment	Kan concentration of MSCP1 (mg/L)	Kan concentration of MSCP2 (mg/L)	Kan concentration of MSCP3 (mg/L)
K1	40	50	70
K2	40	50	90
K3	40	50	110
K4	40	70	70
K5	40	70	90
K6	40	70	110
K7	40	90	90
K8	40	90	110

ISSN: 1472-6750

Treatment	Kan concentration of MSCP1 (mg/L)	Kan concentration of MSCP2 (mg/L)	Kan concentration of MSCP3 (mg/L)
K1	40	50	70
K2	40	50	90
K3	40	50	110
K4	40	70	70
K5	40	70	90
K6	40	70	110
K7	40	90	90
K8	40	90	110

Treatment	Kan concentration of MSCP1 (mg/L)	Kan concentration of MSCP2 (mg/L)	Kan concentration of MSCP3 (mg/L)
K1	40	50	70
K2	40	50	90
K3	40	50	110
K4	40	70	70
K5	40	70	90
K6	40	70	110
K7	40	90	90
K8	40	90	110

Treatment	Kan concentration of MSCP1 (mg/L)	Kan concentration of MSCP2 (mg/L)	Kan concentration of MSCP3 (mg/L)
K1	40	50	70
K2	40	50	90
K3	40	50	110
K4	40	70	70
K5	40	70	90
K6	40	70	110
K7	40	90	90
K8	40	90	110