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Table 2 RT-PCR primers for detecting cDNA transcript

From: Creation and validation of a ligation-independent cloning (LIC) retroviral vector for stable gene transduction in mammalian cells

RT-PCR Primers

Primer Sequence

Bax forward primer

5' CCCGAGAGGTCTTTTTCCGAG 3'

Bax reverse primer

5' CCAGCCCATGATGGTTCTGAT 3'

Catalase forward primer

5' CGCAGAAAGCTGATGTCCTGA 3'

Catalase reverse primer

5' TCATGTGTGACCTCAAAGTAGC 3'

p53 forward primer

5' GAGGTTGGCTCTGACTGTACC 3'

p53 reverse primer

5' TCCGTCCCAGTAGATTACCAC 3'

GAPDH forward primer

5' GACCCCTTCATTGACCTCAAC 3'

GAPDH reverse primer

5' CTTCTCCATGGTGGTGAAGA 3'

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BMC Biotechnology

ISSN: 1472-6750

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