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Table 1 PCR primers for cloning into pBLIC

From: Creation and validation of a ligation-independent cloning (LIC) retroviral vector for stable gene transduction in mammalian cells

cDNA

Primer Sequence

Bax

Fwd: 5' CACACCATCTCAC G GCCACCATGGACGGGTCCGGGGAGCAG 3'

 

Rev: 5' CTCACATTCCAC GTCAGCCCATCTTCTTCCAGATG 3'

Catalase

Fwd: 5' CACACCATCTCAC G GCCACCATGGCTGACAGCCGGG 3'

 

Rev: 5' CTCACATTCCAC GGGTGGCTCACAGATTTGCCTTCTC 3'

p53

Fwd: 5' CACACCATCTCAC G GCCACCATGGAGGAGCCGCAGTCAGATCC 3'

 

Rev: 5' CTCACATTCCAC GTCAGTCTGAGTCAGGCCCTTCTGT 3'

  1. Overhang sequences are bolded, the T4 exonuclease activity termination site guanine is bolded and italicized, and the Kozak sequence is underlined.
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BMC Biotechnology

ISSN: 1472-6750

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