Table 4 Characterisation of antibody fragments generated against Salmonella Typhimurium antigens. n.d. = EC50 not determined
scFv | Target | VH | VL | Yield [mg/L] | EC50 [nM] | EC 50 [μg/mL] | Immuno-blot |
|---|---|---|---|---|---|---|---|
TM228.2.3-B1 | putative dihydroxyacid dehydratase | IGHV3-48*03 | IGLV3-19*01 | 3.8 | 40 | 1.2 | yes |
TM228.2.3-D9 | putative dihydroxyacid dehydratase | IGHV3-23*01 | IGLV1-50*01 | 8.4 | 160 | 4.6 | yes |
TM228.2.3-H7 | putative dihydroxyacid dehydratase | IGHV1-18*01 | IGLV3-19*01 | 12.0 | 310 | 9.4 | yes |
TM228.3.3-A5 | putative electron transfer protein alpha | IGHV1-46*01 | IGLV7 | 4.3 | 78 | 2.3 | yes |
TM228.3.3-C5 | putative electron transfer protein alpha | - | IGLV3-21*02 | 7.9 | n.d. | n.d. | no |
TM228.3.3-D3 | putative electron transfer protein alpha | IGHV5-51*01 | IGLV3-1*01 | 7.6 | n.d. | n.d. | no |
TM228.3.3-F10 | putative electron transfer protein alpha | IGHV1-46*01 | IGLV3-19*01 | 0.5 | n.d. | n.d. | no |
TM228.4.3-A4 | 2,4-dienoyl-CoA-reductase | IGHV3-15*01 | IGLV3-19*01 | 1.3 | 310 | 9.4 | no |
TM228.5.3-G7 | phage tail-like protein | IGHV1-2*02 | IGLV1-47*01 | 7.4 | 310 | 9.4 | yes |
TM228.6.3-A12 | putative dimethyl sulphoxide reductase | IGHV1-69*01 | IGLV3-19*01 | 1.1 | n.d. | n.d. | yes |
TM228.6.3-C5 | putative dimethyl sulphoxide reductase | IGHV4-31*03 | IGLV1-44*01 | 1.5 | 625 | 18.8 | yes |
TM228.6.3-H2 | putative dimethyl sulphoxide reductase | IGHV4-59*01 | IGLV1-44*01 | 2.8 | 625 | 18.8 | yes |