Figure 5

Titration ELISA to analyse the selected anti- Salmonella Typhimurium antigens scFv. 1: putative dihydroyacid dehydratase; 2: putative electron transfer protein alpha; 3: 2,4-dienyl-CoA reductase, 4: phage tail like protein; 5: putative dimethyl sulphoxide reductase. 1 μg purified antigens were coated and detected with a dilution series of purifed scFv. Bound scFv fragments were detected with the anti myc 1-9E10 (1:500) and the goat-α-mouse-IgG (Fab spec.) HRP-conjugate (1:10,000).