Skip to main content

Table 1 Summary of the productivities of the various TAT-Apoptin proteins expressed in the range of E. coli strains

From: Efficient Production of an Engineered Apoptin from Chicken Anemia Virus in a Recombinant E. coli for Tumor Therapeutic Applications

Protein Productivity of E. coli strains (mg/ml) Plasmid
BL21(DE3) pLysS CodonPlus-RP
His-TAT-Apoptin 0.34 ± 0.01 0.82 ± 0.11 0.09 ± 0.01 pET-TAT-VP3
GST-TAT-Apoptin 7.13 ± 1.88 1.15 ± 0.49 2.43 ± 0.98 pGEX-TAT-VP3
His-TAT-Apoptin opt 0.65 ± 0.06 1.20 ± 0.05 - pET-TAT-VP3 opt
GST-TAT-Apoptin opt a 8.99 ± 0.65 6.38 ± 0.18 - pGEX-TAT-VP3 opt
GST-TAT-Apoptin opt b 8.33 ± 0.89 7.84 ± 0.44 - pGEX-TAT-VP3 opt
  1. a E. coli Strains were cultured at 37 °C.
  2. b E. coli Strains were cultured at 25 °C.