Table 1 Summary of the productivities of the various TAT-Apoptin proteins expressed in the range of E. coli strains
Protein | Productivity of E. coli strains (mg/ml) | Plasmid | ||
|---|---|---|---|---|
BL21(DE3) | pLysS | CodonPlus-RP | ||
His-TAT-Apoptin | 0.34 ± 0.01 | 0.82 ± 0.11 | 0.09 ± 0.01 | pET-TAT-VP3 |
GST-TAT-Apoptin | 7.13 ± 1.88 | 1.15 ± 0.49 | 2.43 ± 0.98 | pGEX-TAT-VP3 |
His-TAT-Apoptin opt | 0.65 ± 0.06 | 1.20 ± 0.05 | - | pET-TAT-VP3 opt |
GST-TAT-Apoptin opt a | 8.99 ± 0.65 | 6.38 ± 0.18 | - | pGEX-TAT-VP3 opt |
GST-TAT-Apoptin opt b | 8.33 ± 0.89 | 7.84 ± 0.44 | - | pGEX-TAT-VP3 opt |