Table 1 Sequencing results for the same sample sequenced with either 3,000 ng or 500 ng input DNA
From: Solution-based targeted genomic enrichment for precious DNA samples
Metric | Sample 1A – 3,000 ng | Sample 1D – 500 ng | Average (Std Dev) |
|---|---|---|---|
DNA after prehyb PCR (ng) | 2,734 | 2,625 | 2,432 (541) |
Total sequencing reads | 39,964,814 | 28,476,648 | 37,685,938 (27,502,720) |
% of reads mapped | 97.70% | 98.40% | 94.5% (4.9%) |
% duplicate reads | 56.80% | 44.80% | 53.7% (16.3%) |
% of reads overlapping target | 62.90% | 54.50% | 61.7% (3.7%) |
% of targeted bases covered at 10X | 98.10% | 98.50% | 97.8% (0.5%) |
Avg depth of coverage of target regions | 4,692 | 3,660 | 5,826 (4,200) |