Restriction digestions of the kan gene. PCR products of the kan gene were prepared from the plasmid extracts of DH5a/pDH10 (lanes 1 and 4), RQ7/pDH10 (lanes 2 and 5), and Tm/pDH10 (lanes 3 and 6). Lanes 1-3 represent the PCR products of the kan gene, and lanes 4-6 are samples digested by AgeI. M, 2-log DNA ladder. Analyzed with a 2% agarose gel.