Detection of the transformed kan gene. PCR products of the kan gene were obtained from the plasmid extracts (P, labeled in bold) or the genomic DNA preparations (G) of the recombinant strains. Three RQ7/pDH10 and one Tm/pDH10 transformants, all obtained by electroporation, were examined. Plasmid extracts from DH5α/pDH10 and T. sp. RQ7 were included as positive and negative controls, respectively. Analyzed with a 0.8% agarose gel.