Table 1 Primers used in this study
Primer name | Sequence (5’– 3’) |
|---|---|
GRA2SfiNcoFor | ACGGCCCAGCCGGCCATGGCCGATTTTTCT |
GRA2NotHisBack | TCTGCGGCCGCATTGACTTCAGCTTCT |
GRA2NotHisFor | AGTCAATGCGGCCGCGGTCGAGCACCACCA |
SbfI-His4Back | ACATCCTGCAGGTCAGTGGTGGTGGTG |
FLAGSfiFor | GACTACAAGGATGACGATGACAAGGCGGCCCAGCCGGCCA |
SRS2SfiNcoFor | CGGCCCAGCCGGCCATGGCGCCGTTCAAGT |
SRS2NotBack | TCTGCGGCCGCGGGGGAATCGCCGTTCTCT |
CACC-bx-FLAG-HRV3C Forward | CACCATGAAGATACTCCTTGCTATTGCATTAATGTTGTCAA CAGTAATGTGGGTGTCAACAGACTACAAGGATGACGATGAC AAGGGTGCACTTGAAGTCCTCTTTCAG |
SAG1Forword | TATGGTACCGATCAGAAAAATCACCTCTA |
SAG1Reverse | ATAGAGCTCTCACGCGACGCCAGCCGCTAT |