Expression of recombinant Neospora caninum proteins. MBP-NcGRA2 and MBP-NcSRS2 were expressed in E. coli and purified (A). NcSAG1 was expressed in silkworms and purified (B). Left and right sides of each panel show SDS-PAGE and western blot, respectively. MBP-NcGRA2 and -SRS2 were detected with an anti-His tag antibody, NcSAG1 with an anti-FLAG antibody. Markers 1, 2, and 3 denote Precision Plus ProteinTM Dual Colors Standards, MagicMark XP Western Protein Standards, and Precision Plus Protein™ WesternC™ Standards, respectively.