Table 2 Specifications of the primary antibodies used for flowcytometer (FC) and immunocytochemistry (IC)
Antibody | Dilution | Species | Supplier | ||
|---|---|---|---|---|---|
FC | IC | ||||
CD34 | Hematopoetic percurssor cells and MSCs | 1:50 | 1:100 | Mouse | Sigma-Aldrich® |
CD45 | Anti-bone marrow lymphoid cells | 1:50 | 1:100 | Mouse | Sigma-Aldrich® |
CD44 | Cellular receptor for hyaluronic acid reactive with bone marrow nucleated cells | 1:30 | 1:50 | Mouse | Sigma-Aldrich® |
CD90 | Anti-THy1 antigen | 1:30 | 1:50 | Mouse | Sigma-Aldrich® |
CD105 | Anti-endoglin | 1:50 | 1:25 | Mouse | Sigma-Aldrich® |
CD29 | Anti-integrin β1 | 1:50 | 1:25 | Mouse | Sigma-Aldrich® |
CD73 | Anti-nucleotidase | 1:25 | 1:25 | Mouse | Sigma-Aldrich® |
Anti- Vimentin | Anti-vimentin clone V9 | 1:50 | 1:500 | Mouse | Sigma-Aldrich® |
Anti- cytokeratin | Anti-Pan cytokeratin clone PCK-26 | 1:100 | 1:100 | Mouse | Sigma-Aldrich® |
GFAP | Glial fribrillary acidic protein | 1:50 | 1:500 | Mouse | Dako® |
CXR4 | CXC chemokine receptor 4 | 1:50 | 1:150 | Mouse | Calbiochem® |
SNAP-25 | Synaptosomal- associated 25 kDa protein | 1:50 | 1:100 | Mouse | Prestige Antibodies™ |
Anti-N200 | Meurofilament 200 Kda | 1:100 | 1:100 | Mouse | Sigma-Aldrich® |