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Figure 4 | BMC Biotechnology

Figure 4

From: Isolation and characterization of Wharton’s jelly-derived multipotent mesenchymal stromal cells obtained from bovine umbilical cord and maintained in a defined serum-free three-dimensional system

Figure 4

Photomicrographs representative of the morphological appearance of chondrogenic, osteogenic and adipogenic differentiation of bovine-derived UC-WJ cells at P40. For each differentiation protocol, undifferentiated cells were kept as controls ( D, E, F). A) Presence of calcium mineralization after 20 d of induction Alizarin Red staining; B) Presence of acidic proteoglycans were observed after 1 week of chondrogenic differentiation by Safranin O staining; C); adipogenic differentiation after 15 d showing lipid droplets stained with Oil Red (scale bar: 20 μm) ( D); after neurogenic induction of 2 week a neuronal-like morphology was visible under immunofluorescence microscopy positive N200 cell marker ( E) (scale bar: 20 μm).

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BMC Biotechnology

ISSN: 1472-6750

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