Table 1 The top patterns of abnormal sequences detected in 172, 229 Pinus taeda ESTs generated from UGALGB

1

5'

N,3TSS-3,N,…,3TSS-3,N

27426

30.74%

Single or multiple poly(A)

fragment(s).

Additional file 1: Figure S1 A

2

3'

(N,)5TNS-4,N

7707

9.28%

XhoI site (CTCGAG)

is replaced by C.

Additional file 1: Figure S1 B

3

3'

(N,)5TNS-5,N

6814

8.21%

The vector fragment 2 (VF2) has

been identified, without adjacent poly(T)

tail and XhoI site detected.

Additional file 1: Figure S1 D

4

5'

N,3TSS-5,V

1414

1.59%

The vector fragment 2 (VF2) has

been identified, without poly(A)

tail and XhoI detected.

Additional file 1: Figure S1 E

5

5'

N,5TNS-1,N

1386

1.55%

3′-like terminus, i.e., poly(T)

and XhoI site, without the vector

fragment 2 (VF2) detected.

Additional file 1: Figure S1 H

6

3'

5TNS-2,N

1238

1.49%

XhoI site and the vector fragment

2 (VF2) identified, without a poly(T)

tail detected.

Additional file 1: Figure S1 F

7

5′

N,3TSS-5,N

873

0.98%

The vector fragment 2 (VF2) has

been identified, without adjacent poly(A)

tail and XhoI site detected

Additional file 1: Figure S1 G

8

5'

N,3TSS-4,V

800

0.90%

XhoI site (CTCGAG) is replaced by G.

Additional file 1: Figure S1 C