Table 1 Details of the primers used in the LAMP-BART and RT-PCR amplifications
Primer Type | Orientation | Target (5' base) | Primer Sequence (5'-3') |
|---|---|---|---|
Displacement | sense | ADH1 (7) | CTTTGGATCGATTGGTTTC |
Displacement | antisense | ADH1 (287) | CCCAAAATTACTCAACG |
LAMP | sense | ADH1 (116) | GGTGATCAAGTGCAAAGGTCTTTTCATAAACCAAGATTAGTCAGATCAAG |
LAMP | antisense | ADH1 (94) | CCCCTCCGCAAATCTTCGAACAGTTTTGTAACTGGTGAAGGACTGAG |
LOOP | sense | ADH1 (68) | CGCCTTGTTTCTCCTCTGTC |
LOOP | antisense | ADH1 (136) | CCAAATCATCCACTCCGAGAC |
Displacement | sense | CaMV-35 S-p (7214) | AGGAAGGGTCTTGCG |
Displacement | antisense | CaMV-35 S-p (7404) | ATAAAGGAAAGGCCATCG |
LAMP | sense | CaMV-35 S-p (7317) | GTCTTCAAAGCAAGTGGTTTTGGATAGTGGGATTGTGCG |
LAMP | antisense | CaMV-35 S-p (7296) | TTCCACGATGCTCCTCGTTTTCCTCTGCCGACAGTGG |
LOOP | sense | CaMV-35 S-p (7274) | TCCACTGACGTAAGGG |
LOOP | antisense | CaMV-35 S-p (7350) | GGGGTCCATCTTTGGG |
Displacement | sense | NOS-t (1850) | CGCGATAATTTATCCTAGTTTG |
Displacement | antisense | NOS-t (2053) | CGTTCAAACATTTGGCAAT |
LAMP | sense | NOS-t (1962) | GCATGACGTTATTTATGAGATGGGTTTTCGCTATATTTTGTTTTCTATCGCG |
LAMP | antisense | NOS-t (1947) | CATGCTTAACGTAATTCAACAGTTTTTGAATCCTGTTGCCGGTC |
LOOP | sense | NOS-t (2007) | GATTAGAGTCCCGCAATTATAC |
LOOP | antisense | NOS-t (1925) | AAATTATATGATAATCATCGCAA |
PCR | sense | ADH1 (1297) | AATTTTGGGGAAAGCTTCGT |
PCR | antisense | ADH1 (1369) | TTCACCACGATTGCAGGATA |
PCR | sense | CaMV-35 S-p (7133) | GATTCCATTGCCCAGCTATC |
PCR | antisense | CaMV-35 S-p (7215) | CAACGATGGCCTTTCCTTTA |
PCR | sense | NOS-t (1854) | TCGTTCAAACATTTGGCAAT |
PCR | antisense | NOS-t (1885) | AAGACCGGCAACAGGATTC |