Analysis of GLP-1 mRNA expressions using quantitative-PCR. GLP-1 expressions in five isolated L-cells and STC-1 cells (as a control) were measured. All the isolated cells expressed more GLP-1 mRNA than the control cells. The BioRad CFX Manager software was employed to analyse GLP-1 expression data. The mouse β-actin and β-2 microglobulin (β2 m) mRNA levels were used to normalize the values. Error bars indicate standard deviation, p < 0.05.